Watch the replay of our webinar below along with the transcript.
The webinar generated numerous insightful questions from attendees, many of which we could not address during the session due to time constraints. Her are comprehensive responses to all the queries that remained unanswered.
Will you offer hardened off in a tray solution that’s greenhouse ready?
We are currently exploring different product form factors, including a more hardened off clone.
When keeping VPD in the correct range, is leaf surface temperature taken into account?
For the purpose of this research, these numbers focused on ambient air temperature in the cultivation space rather than leaf surface temp.
Have you seen changes in performance since moving to rockwool for Conceptions rooting TC phase?
We have seen more robust roots since switching to the rockwool plugs.
I know you talked about meristemming to remove pathogens but its still possible to contaminate clones after merristemming, even with tissue culture. How do you guys go about testing to guarantee no spread of virus/pathogen to customers?
We deploy an incredibly robust facility hygene testing protocol through the guidance of our in-house pathologist Dr. Maria Rojas. The protocol involves weekly air plates, surface swabs, and sample plating to both validate sanitation efforts and the integrity of material being shipped to customers. Plant stock is routinely tested for endogenous pathogens/viroid, and plant stocks are routinely refreshed with new meristematic initiations to de-risk the possibility for latent buildup accumulating.
*Please note, the transcript is auto-generated.
06:00
So people ask us all the time kind of what is tissue culture. And in summary plant tissue culture is a technique used to propagate and grow plants under aseptic conditions in a controlled environment. The process basically entails the cleaning and subsequent propagation of plants in vitro from the tissue of a mother plant it’s been in mainstream use since about the sixties, and people have been doing tissue culture now around a hundred years.
06:35
Yeah, and just to draw the line there, too, between a couple of different techniques and tissue culture that you may have heard of before. There are 2 techniques that we utilize in particular, the first one being a meristem initiation and the second one being nodal propagation, so as it pertains to our operation meristem initiations are a little bit more take a little bit more time than nodal propagation, and that’s because we are starting a culture from a really small group of cells known as the meristem. We are isolating the mare stem itself. propagating that group of cells into a plant lit, and then subsequently, taking cuttings off of that plantlet to propagate a larger population. And the subsequent subsequent propagation. Of a meristomatic initiation. Is no is known as what’s called nodal initiations, where basically rather than excising the mare stem itself. We are simply taking a cutting. So a piece of the stem with some mare stematic cells attached to it and propagating all clones or cultures through that technique. So we will develop a clean population using meristem, validate that population through rigorous pathology and virology testing and then ultimately utilize nodal propagation to propagate that clean population more effectively. So just in case y’all have heard either of these terms used in the past, we actually do utilize both processes to create an effective and validated product from the ground up.
08:30
So the other question we’re asked a lot is what? Why tissue culture for cannabis? And ultimately the widespread commercialization of cannabis has had a number of challenges with pest and disease pressure. Particularly hoplite and thyroid over the last number of years. It’s really tissue culture is a good solution for kind of modern day commercial cannabis cultivators that it does a number of things. It allows a hygienic means of high throughput propagation. There’s long term, long term, stable genetics, preservation. clean dissemination of novel genetics, and ultimately more reliable starting material that owners, operators can really count on. So now we’re kind of getting into the main part of this webinar. And this is where we’re going to be talking about acclimating Gen. 0 clones and kind of the parameters around that. And we found that that these 4 parameters, humidity, light intensity, East, Ec. Strength, and Vpd are really critical to the success of a smooth acclamation where you’ll get. you know all your plants transitioned. Healthy and effectively. We call this the hill technique. It kind of came as an acronym for high, low, low, which you can see in this chart of the levels. starting with high humidity, low light intensity, low Ec, strength and low. Vpd. now we’re gonna go into this in a lot more detail on these following slides. But I just wanted to give you guys kind of an overview of what we’re going to be focusing on for this one something important to note is that we’re also this also focuses really on the first 7 days of acclamation. So post post 7 days, you can really start ramping things up and we’ll talk about that a little little further down the road. So next slide, please. We’re gonna talk about the importance of acclamation, visual indicators, what visually, what are, what’s the difference between successful acclamation and unsuccessful acclamation.
11:04
And we think these visual indicators are really important to cultivators, because ultimately based on these parameters that we draw out for y’all using the HLLL technique. Ultimately. Everyone’s cultivation environment is gonna be a little bit different. I don’t need to speak to the nuances between cultivation facilities. I’m sure we all understand that there’s gonna be a little bit of difference from one operator to the next. And ultimately these visual indicators are gonna serve the purpose of giving y’all some relativity to understand what to expect based on your parameters. So really, just wanna give you all the resources beyond environmental parameters to have some expectations for what you should see visually with the tissue culture clones as they acclimate.
11:56
So this is what successful acclamation looks like. Healthy, lush, green growth. nice turgidity. you know. perked up plants like like we like to see well developed root system. I don’t know. These are on the left. This is from a grow and a greenhouse in Humble county. They did a great job of the large scale acclamation
around 5,000 plants. The one on the right is is from an indoor grow down in Mendocino County. Smaller number of plants. you know, there are many ways to to get to a healthy acclamation, and we’re gonna dive into that a little bit more. So this is what unsuccessful acclimation. Looks like you. You guys can see the the yellow leaves, the red stems, the stress in these photos on the left. and then on the right. You see the the wilting, just major stress or root colonization, etc. So let’s get into the humidity. This is probably the most critical parameter for that first week. I tell people the target around 80% humidity for the first 4 to 5 days, and then you can slowly taper that down. During the latter part of the week. The easiest way to do this is through regular cloned dome. It’s probably the easiest and most reliable way to achieve that 80, especially if the room, the environment outside that dome is is a little drier, and you know, nice and warm. So what I say is, yeah, start high and then slowly taper down after 4 or 5 days, get it down, maybe to that 70% range. When you, when our plants arrive to your facility. They’re in a sealed pack, and that inside that pack it’s around 95% humidity. And the idea is that we gently want to taper that humidity level down as this process moves along. Alex, is there anything you wanted to add to that.
14:28
No, definitely just to your point, Sam, about the unique form factor that our packs are coming in. It’s a sealed pack of about 48 plants and you know, relative to most cultivation environments, 80% might seem a little bit high, but it really is. With regards to relativity. Where the packs are coming out of from our lab environment. Into Sam’s Point. The cloned dome is gonna be the easiest way to achieve that and begin the plants with as gentle of a start as possible.
14:57
And then, you know, adding to that, I would say, If if you’re doing a scale that is clone domes don’t make any sense. For example, if you’re ordering 5,000 plants. you know, in that situation. a misting system or a humidifier like a commercial humidifier, those work work well to get the humidity where you want in that target range. I’ve seen both all, you know. All 3 versions do very well with acclamation, but the easiest and simplest way certainly is the clone dome and perfect for medium to smaller cultivation. So next one. and of course you measure humidity with a high grammeter. I know all cultivators are probably pretty familiar with that instrument. so light intensity is the next big parameter we’re going to talk about, and when the plants leave our lab. They’re used to about a hundred 50 micromols of light intensity. So what we want to do is just kind of gently, so step that up. I tell folks often just target around 200 micromols the plants will be good. Make sure you use a par meter to to do this. Not a lux meter par is is really what the plants are going to respond to. It’s that 400 to 700 nanometers. It’s really the ideal level of lighting right around, you know, anywhere from 1 70 to 2 50. I’d say, if this is your first time, maybe shoot to for the lower end of that number, maybe like 1 70 to 200 to just guarantee a nice, steady, easy acclamation for those plants. You know, too much light can stress them out. What we really want to focus on is is letting those roots spread out and settle in to their new substrate. Lastly, I would say, you know, T. Fives work great leds the clone strips work great or if you’re in a greenhouse or hoop house some 50% shade cloth over the top. Good to go.
17:24
Yeah, I think the only thing I wanted to add to that is, you know, the the lighting scheme being 18 6. Really not doing anything special with the plants to you know, change that timeframe or anything keeping it simple is the best route to take it.
17:42
Yeah
grade.
And then you can see on the next slide. There’s a couple of par meters there. Apogee. Certainly the gold standard of par meters. Very nice works, great and then on the right, you can see a a cheaper, a cheap version. It’s about a fifth. The cost of the Api G unit. If you don’t have one at your farm, I highly recommend getting one, and we certainly have a few loaners we can send out to if you just need one for your acclamation
with our plans. So Ec strength. And and this is important, because the last thing we want to do is put extra salt stress on those roots right? So we want to keep them searching. reaching outward for for nutrients and moisture, and we found better success, starting with a lower level. Lower strength fertilizer! Then you’re probably you would feed a a new traditional clone. I like to tell folks, you know. Probably one and a half is a good place to start with. The Ec. Maybe if you’re in Rockwell you could bump it up a little bit higher into that, like 1.7 1.8 range, and if you’re in coco or and or maybe an amended engineered soil, mix on the lower end is probably fine. 1, 2 to 1, 5, but you know 1 5 is a good starting point. Certainly, if it’s your first time. and then I would certainly focus on using a just a balanced veg fertilizer. Not anything. Fancy. Maybe you know 2 part basic type fertilizer. And and we can make recommendations for specific specific fertilizers, organic or mineral based. Be happy to do that. Start on the lower side. I know a lot of nutrient manufacturers want you to hit them with 3.0 out the gate. But with our plantlets, that’s that’s going to be too much for them, so start low. Take it easy. It’s not. It’s you’re not going to be limiting the the speed at which they grow with a little less fertilizer. It’ll actually improve things further down the road. So you have a nice strong start, which down the road will translate to healthier and healthier plants.
20:20
Yeah. And to Sam’s point about, you know the the being worried about lagging the growth of the plant by not hitting it with, you know, a strong enough fertilizer. This the first 7 days. This acclamation process that we’re describing is really about derisking any unnecessary abiotic stress that we could be putting on the plant with regards to Ec. We really don’t want to be over feeding them, you know, the 7 Day acclamation process really serves as a gate after you know you make it through that gate. The Tc. Clones are going to be much more resilient to fluctuations in humidity and temperature increased Ec. Strength, etc. But the goal again of this.
21:09
But what.
21:10
First 7 days of acclamation process are to really derisk any unnecessary plant loss. It’s not about getting them going as fast as possible. It’s about getting them going at an appropriate pace that isn’t lagging too much. but ultimately is derisking any unnecessary abiotic stress on the plants so that we can set strong foundation for the Tc. Clones perform all the way through the cultivation process. The last thing we want is to add a little bit of abiotic stress, excessive abiotic stress on the front end resulting in maybe some dampened rooting and you know, giving you any kind of variance moving into veg. We want all these plants performing in tandem with one another as uniformly as possible, and again derisking any unnecessary abiotic stress is definitely the goal. Here, for the first 7 days of the acclamation process.
22:11
So that really brings us to the next slide, which I, which is epd. as we all know, Vpds being a lot being talked about a lot more in today’s cultivation schemes. It’s really the difference between how much water can be held at a certain temperature versus how much water is in the air at that. At that temperature. Dialing this in will really get your plants transpiring at the appropriate rate for the stage they’re in. We’d like to target like a point 60 to point 8 9 Bpd, that seems to be the sweet spot of you know, getting the right level of humidity. you know, at the right temperature, certainly up above 75 degrees when the lights are on or during the day is ideal. they can certainly take a you know, lower temperature at night, you know. 10 degree temp. Temperature. Differential is fine. For example, if you’re you’re cultivating in a greenhouse, and it’s dropping into the sixties. You’ll be fine. But yeah, keeping it nice and humid. Nice, warm and humid is is really key. I like to tell folks, you know, 80 80 degrees 80% humidity kind of is, it’s an easy thing, easy number to remember. Alex. What else would would you like to add to this one.
23:50
Yeah, really, to the point that you just made Sam about the 80 80 being a sweet spot for recommendation for Vpd, we do have a photo of a Vpd chart on the following slide, Britt, if you wouldn’t mind transitioning over where, if we look at the parameters at 80% relative humidity and 80°F. We’re finding ourselves right in the middle of that point 6 to about point 8 9, find ourselves at about a point 7 one vpd level. So really smack dab in the middle of that optimal range. anything here highlighted in the blue is gonna fall within that optimal range, but just drawing the point here that the recommendation recommendation of you know, setting your goal at about 80 80 is gonna put you smack dab in the middle of the optimal range. So doing your best to maintain those parameters is really gonna be conducive to your success and just wanted to go over a couple of things with regards to Vpd levels that are a little bit too low. Vpd levels. That are a little bit higher. Basically, we want the plant transpiring at an appropriate rate. That’s gonna allow for adequate dry back. Send the roots searching for, you know, moisture and nutrients. But essentially isn’t having them search too hard. Right? If we’re drawing the plant back too quickly. We’re getting into an issue where we’re gonna have to begin modulating our fertilization, cadence. etc. So really, during these first 7 days, if your Vpt levels are appropriate, you have appropriately charged your substrate before transplanting, we think there should possibly be one watering event towards the end of these 7 days. As you notice, substrate, begin to dry back. Notice a little bit of loss of rigidity in the plants as they begin to search for that new moisture. But keeping your Vpd. And this optimal range is really gonna give you an easier time when it comes to your watering cadence. During those first 7 days. And ultimately drying out you know, the most developed root mass that you can during this acclamation process. So to Sam’s point about pushing the plants. The vapor pressure deficit is definitely one of those things that we wanna try our best to follow guidelines here. Put you smack dab in the middle on the optimal range. And we really, for the first 7 days, don’t wanna deviate too much from that optimal range. Yeah.
26:34
Exactly. So. There’s there are a few tips and tricks we like to recommend that we found. To just add, optimize your acclamation, get the highest rates get the widest, fuzziest, healthiest roots growing and one of the first things I’ll often recommend is using a biological inoculant essentially to bioharden the plantlets you can see a few inoculants that we like here on the left hand side th. These were great. Some are are more bacillus and bacterial based others, or. like the root shield, is certainly Trigoderma based anything. That really just like protects the plants out competes, you know bad bad funguses and bacteria. That’s what you want, and that’ll really get you the the widest, fuzziest roots. Let’s see, in addition. the you know clone domes. That’s, you know, great trick. Easiest thing. Getting a good watering cadence and using an intermediate size substrate before planting. I certainly recommend that it’s just easier to manage the dryback of a smaller substrate rather than going into something big right away, you know, for example, if you’re going and you know your end goal is to go into a Hugo block. Rockwell, Hugo block I would recommend going into, you know, a 2 inch or one and a half inch plug, first getting the roots busting out of that before going into the the bigger substrate. It’s just gonna make for it’s just easier management requires a little less skill than going into something large right away. Whether it’s a Hugo block or a one or 2 gallon cocoa pot. What have you stepping it up will will make your life easier that I can assure you. And then, you know, pre saturating your media with fertilizer charge, that’s that’s absolutely key. Particularly if you’re using an inert substrate and and making sure that it’s nice and pre saturated, mixed up so that you’re not coming across dry pockets or uneven moisture levels. Just just keeps your whole cultivation scheme. Just more even in general.
29:20
Definitely a a a tip more pertaining to like traditional agricultural practices. Right, Sam, pretty saturating your media to ensure, especially at a larger scale of production the most consistency possible when we’re dealing with the tissue culture Clones and you know, rearing them to enter into the traditional cultivation. Space. Things like root, mass development, and vigor of growth are in parallel with traditional clones. And really, the the pre saturation of the media is, gonna allow you to add that much more consistency to your operation, right? If they’re all drying back around the same time. Your watering events are gonna be a lot more convenient to schedule and then ultimately, that watering cadence
that we spoke of is gonna be a lot easier to dial in if you’re starting with evenly saturated material and to Sam’s point about the use of an intermediate substrate definitely, a lot easier to regulate dry back in a smaller sized pot. Than, for example, going straight into a one gallon cocoa bag where it’s still possible to have great success. Going straight out of our packs into a one gallon cocoa pot definitely a little bit more difficult to dial in. Per, your facilities. Specific parameters, right? So this suggestion of an intermediate block is just so folks can understand how these tissue culture clones are behaving in their specific space. And ultimately, if folks do find the opportunity to sort of round off some of these corners. And go into a larger substrate than what we’re recommending. We really think that that process is well informed. By going into an intermediate, at least your first couple of orders to understand what the routing rate looks like. What your watering cadence is gonna look like in this specific pot size? And then ultimately, what you choose to do with that information is going to be dependent on your specific operation. So I also wanted to touch base on what Sam had mentioned about the bio hardening and how that you know, with regards to tissue culture, clones is of particular importance. The environment that the Tc Clones are coming out of is very sterile, right? We’re also utilizing Rockwell as a substrate. So the environment that the plants are being cultivated in is very sterile and not biodiverse right? And, as we all know, moving into the traditional cultivation environment, it’s quite biodiverse not necessarily just with regards to pathogens, but just biology itself, right? Native soil, biology things like that. And the inoculation step for the Tc clones is really to bolster the plants with beneficial microbes in that sterile rock will substrate such that when they are exposed to simply native biology. at, you know, traditional cultivation facilities, they don’t go through any type of shock, or any. You know, there’s no perturbances to the colonization of the Rockwell substrate by native biology. The inoculation really serves the purpose of sort of pre loading the substrate with beneficials, so that the plants shock when it’s being introduced to this more biodiverse environment is mitigated as much as possible. So and then the last thing the last tip and trick that I wanted to touch on that’s especially important with regards to ordering our tissue culture clones. And this acclamation period we’re describing is planning and logistics surrounding intake. And you know, allocating space and things like that for this acclamation process. We really don’t wanna put a bump in the road when it comes to the efficiency of your cultivation process. So understanding that this 7 day acclamation period is a reality and just planning your intake, based on that right, especially for the folks working indoors completely understand the turn and burn nature of indoor facilities. You guys are really chasing those 5 flips a year. And just guaranteeing that you guys are in taking your order with an appropriate amount of runway to get this acclimation process complete. such that by the time you guys pull your room down, sanitize it and get it ready for new plants to go in, that the Tc. Clones are well acclimated and ready to go right the same way. You were forecast for your traditional cuttings to be rooted and prepared to move into the vetch cycle. We’re calling for some similar planning and logistics with regards to intaking the Tc. Clones, getting them into that secondary substrate. starting to taper down environmental parameters, and ultimately having the Clones well rooted, acclimated, and ready to go by the time you’re ready to fill that room. So working with our sales team, and Sam and I as customer success managers to really dial that off. Take in and make sure that you have all the space equipment, and information that you need to acclimate and prepare your space to receive the Tc. Clones. Sam, did you want to add anything else to that?
35:14
I think you covered the things I was gonna add in. So I think we’re good. Appreciate it.
35:20
Awesome.
35:28
Right now we have some time for some questions. Thank you guys. For all that great information. I have a question. If you guys could speak on this a little bit? Can you talk a little bit about how is this information based on research that you’ve done in the lab, or is it based on what you’ve seen out in the field, as you guys are, our customer success team.
35:51
So definitely. This is all based on observation that we’ve made both internally as well as with you know, observations being made at customer facilities. So we do have internal partners that have helped us lay out these guidelines and ultimately working with customers who have both adhered very closely to these guidelines, and have, you know, done their own due diligence to understand the capacities of what? What is possible? What the plants are capable of? In their own facilities. But ultimately, the HLLL protocol is the product of huge amounts of customer feedback as well as our own observations that we’ve done internally with partners. Sam, did you want to add anything to that?
36:53
We definitely wanna take a multi faceted approach, because, bearing in mind, you know, when it comes to testing. And R. And D, we talked a little bit about the nuances between cultivation facilities. and I’m not necessarily talking about an indoor cultivator compared to a greenhouse cultivator or an outdoor cultivator. I’m more talking about the nuances between one indoor cultivator to the next indoor cultivator, one greenhouse cultivator compared to the next greenhouse cultivator. It’s gonna be some differences right from the nutrient line that you’re using to your environmental parameters, the lighting, etc. as much as we rely on internal partners to you know, understand and record some of this data. We do absolutely realize that there are a whole you know, whole bunch of unique systems to trial these plans through and that’s why we really appreciate working with our customers to gain feedback from them about both their successes and their difficulties. So, taking all that into account when laying out these guidelines was incredibly important to us. So that we can have, you know, the most homogenous solution for cultivators looking to utilize the tissue culture technology as possible.
38:12
Awesome. Alright we have some audience questions. I noticed your plans are smaller than traditional clones. Can you talk about that a bit?
38:24
Sure. So wh when they arrive at at your facility, they’re probably some of the smallest clones you’ve ever seen. That being said I’ve done side by side trials, and they catch they will catch up with a traditional clone. under the right conditions. So I wouldn’t be discouraged by really their size, what they’re they just have so much potential in there. And once they really root into that substrate, the plants take off. and you’ll you’ll notice wider leaves, thicker stems, denser. tighter, inner noodle spacing. You know things that that make a difference when it comes to yield and plant performance when compared to a traditional clone. So I encourage that anyone to do side by side themselves and and see for themselves. But at the end of the day while they look small. And it’s it’s hard to imagine that they could perform and and grow faster and actually surpass a traditional clone. They will.
39:37
Some. Okay. Can you explain how tissue culture will lead to more consistency in a grow.
39:49
I think the biggest value proposition definitely. Outside of taking any economic traits into consideration. About, you know, growth rates and stuff like that is really derisking. The influence of both pests, pathogen and disease. Right? We are all familiar with in taking batches of third party Clones. having a group of those clones that are have been compromised by one issue or the other. having to quarantine that group of Clones deploy an Ipm strategy and sort of crossing your fingers that that group of quarantined Clones is going to catch up or or stay at pace with the healthy clones that you received the big value proposition that we’re offering is that all of our Clones are going to be coming to you devoid of any pests or disease. And what that allows for on the front end. Is sort of this even treatment of your lot of plants right? There’s not going to be a portion of them that we’re going to require. Excessive Ipm application. There’s not going to be a portion of them that you’re going to need to quarantine and set aside from the rest, and possibly a different room in a different environment under different lighting. We really think absolutely any variation, you know, in these first couple of stages of the cultivation process really lead to more bumps down the road. And starting with consistent pest and disease free clones. Really is our a a stronger suit of our our value proposition here in that, you know. Starting with a consistently healthy batch of clones gonna lead to consistent health down the line.
41:34
Yeah, I mean. you nail the acclamation. You’re gonna have a nice, even canopy. You won’t. you know, when I was back when I was growing traditional. Clones, I’d always notice, you know, a decent number of of plants that we’re kind of runty, or you know, didn’t want to take off like the others, but from my experience with the Gen. 0 plans. I’ve seen it for myself. The the consistency is unlike
what I’ve ever ever seen before in the past. So the main key, though, is, is nailing that acclamation so that everything downstream goes without a hitch. and you get the results you’re looking for ultimately.
42:22
Awesome. Okay? Great. Let’s see what intermediate substrate do you recommend for indoor Rockwell operation?
42:34
So certainly with a indoor rock wall operation. And I kinda touched on this a little bit earlier. But you know, a 2 inch a okay plug. one and a half and using maybe a dibbling tool. I’ve even used my finger to to make holes in the rock wall, and it’s it’s worked fine. but I I would go into something small to start. I’ve also seen some cultivators go into a 4 by 4 by twos very successfully, and then transition those on top of a slab or a Hugo block that also works well, just just not going right into the slab or right into the the Hugo block out the gate is, gonna make your life easier. It’s gonna be easier on the plants. What I’ve noticed sometimes, particularly in Rockwell, is that and I can like in a Hugo block. The top half is gonna drive back significantly more than the bottom well and and getting that the the moisture level, how you want as even as you want is harder in that substrate for acclamation. So yeah, certainly encouraging, starting small and going into something bigger really is is the way this is going to be the easiest way to have acclamation, success.
44:04
Awesome. Okay. Next question. can you explain? What do you mean by generation? 0 clones?
44:17
So generation 0 cutting refers to specimen that is coming right out of the in vitro growth that we observe in Tc and propagating a cutting from you know, coming out of that tub so to draw the line here jen one cutting would be a cutting taken off of one of these generation 0 plants so kind of referred to as tissue culture clones. For the sake of motherstock. we would be sending out a generation 0 clone to be propagated. And then any cuttings taken off of that generation, 0 clone would be considered a generation. One clones. So just some scientific jargon there. Gen. 0 versus Gen. One
45:10
Wolf. We like science. Okay, do you have any examples of successful transitions from traditional propagation to Tc.
45:27
So I feel like I’m a good example of of making that transition from traditional plants to to Tc, ultimately. I’ve I’ve had better yields healthier plants, more consistency. bigger, wider leaves. I mean significantly better yields I can look at my metric data year over year, and it made it a surprising difference in what I was able to pull down running traditional plants versus a gentero plant. So but ultimately you’re you’re you’re looking at at at bigger, healthier plants. That’s been my experience.
46:16
And what? What type of growth do you have, Sam? Can you tell.
46:20
So in greenhouses and environments, these are these are things I’ve noticed.
46:30
Cool. The reason I ask is, the next question is, how do you best recommend? I achieve high humidity as an outdoor grower.
46:41
So as an outdoor cultivator. But the the easiest approach for this is, if you have a small small hoop house you can do your acclamation in use. The domes use a dome and a hoop house with some shade cloth. It’s really that simple and after a week or 10 days, you can start really transitioning them to the from the shaded area to to the full sun. And yeah, you’ll have a great start.
47:16
Alright. Another question about humidity. Have you ever seen problems with molds in that high of humidity.
47:26
So I would say the the window of humidity is is really pretty short. When we’re talking about that 80% you’re talking about 4 to 5 days. with events all the way open on your clone dome. which is what we recommend. The humidity doesn’t become an issue where we’re we’re seeing any type of of new pathogens developing in that short amount of time.
47:57
And if you all are noticing, you know, an excessive amount of condensate building up on your your clone dome same way you would treat traditional clones right. If you need to burp the dome, wipe down that excessive content. Sit same thing applies right to Sam’s point about that range being sort of on the the shorter end of the the acclamation process. Doesn’t. Doesn’t mean that you you’re not able to, you know. deploy traditional clone care right? If you see excessive condensit. By all means you’re able to take that dome off for a couple of minutes.
Wipe off that excessive common set, and de-risk the chance of any you know, any kind of pathogen or mold finding a home in that clone dough.
48:44
8. Okay? And let’s see. will these techniques work on any tissue culture plants?
48:55
I would say in general, yes. but certainly we’ve had the most experimentation and experience with the the plants from conception nurseries. So we’re most familiar with that form factor. but in general these would likely work well with any tissue culture plants that.
49:18
Right, I think we should take maybe one more question. Let’s see, what what kind of help can you give farms for trying to shoot culture for the first time.
49:32
So for a farmer trying tissue culture for the first time. The main thing I would emphasize is, it’s not complicated, and we’ve gone through a lot of scientific detail in this in this webinar. But I wouldn’t be discouraged by getting every single thing absolutely perfect. And and if you don’t. It’s not gonna work like that’s not the case. There, we we target all these levels. This is these are all like ideal case scenarios. But you can. You can have success without getting some parts in in perfect. It’s it’s actually pretty simple, like, you know, the clone dome, low light. you know. High humidity with that with the domes and night, you know. Nice and warm it’s it’s not. It’s not as complicated as it it may might seem like anyone who’s who’s been cultivating cannabis for any like, you know. A decent length of time will comfortably to comfortably handle this once they get their plans, I would say.
50:48
We eat. Alex, and you you guys are out there helping first timers as well. Right definitely.
50:55
Split.
50:57
Right? Yeah, we were.
50:59
Do site visits for first timers, and we can show you hands on. You know, every cultivation site’s a little different. So we like to work with cultivators, to find the easiest, best approach kind of customized for for their scene, and what they have going on at their place.
51:20
Yeah, definitely to Sam’s point about, you know. serving as a resource beyond just the initial acclamation process. We would love to be, you know, developing longstanding relationships both with operators and their crew, to better understand. You know what solutions are gonna best suit them. So, starting with this acclamation process as a a building point, and ultimately carrying this relationship through all the way to harvest and seeing how we can best service your guys grow.
51:51
And I think I think one thing that we do really well is helping people get ready to receive the clones, so that they have all the tools they need to transplant and acclimate correctly. Right.
52:06
Yeah, like we talked about earlier. If you have the need for par sensor, ecph, meter, etc. We’re happy to provide those tools anything conducive to customer success. We’re happy to provide from, you know, a brief phone call to go over this acclamation process and some tips and tricks all the way through to showing up at your facility. Alongside your first order. And really kind of fight. Loving this experience for you guys.
52:38
Alright. With that I think we’ll close. So if you have any further questions for us, or if you’re interested in learning more about conception you can contact us at grow at conception nurseries.com. We will have a one page guide to this technique that we’re gonna send to all the in attendees. Along with the recording. So thank you, and look out for more upcoming webinars from conception.
53:04
Thanks. Y’all, thanks very much for coming. And to Britney’s point any further questions or anything. Don’t hesitate to reach out we’re open books here, happy to talk whether it be about acclimation or just the tissue culture technology itself. Both Sam and I are really excited to be engaging with customers on this topic, so please don’t ever hesitate to reach out.
53:22
Absolutely. We’re always here to make sure you guys succeed. There’s nothing we want more than to see you win.